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yeast lysate

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  • Immunoprecipitation — (IP) is the technique of precipitating a protein antigen out of solution using an antibody that specifically binds to that particular protein. This process can be used to isolate and concentrate a particular protein from a sample containing many… …   Wikipedia

  • MRNA display — is a display technique used for in vitro protein, and/or peptide evolution to create molecules that can bind to a desired target. The process results in translated peptides or proteins that are associated with their mRNA progenitor via a… …   Wikipedia

  • Protein — Proteins are large organic compounds made of amino acids arranged in a linear chain and joined together by peptide bonds between the carboxyl and amino groups of adjacent amino acid residues. The sequence of amino acids in a protein is defined by …   Wikipedia

  • Fungal prions — have been investigated, leading to a deeper understanding of disease forming mammalian prions.Prion like proteins are found naturally in some plants and non mammalian animals. Some of these are not associated with any disease state and may… …   Wikipedia

  • Nuclear run-on — A nuclear run on assay is conducted to identify the genes that are being transcribed at a certain time. Cell nuclei are isolated rapidly, and incubated with labelled nucleotides and the results are hybridized to a slot blot, which is then exposed …   Wikipedia

  • Methods to investigate protein–protein interactions — There are many methods to investigate protein–protein interactions. Each of the approaches has its own strengths and weaknesses, especially with regard to the sensitivity and specificity of the method. A high sensitivity means that many of the… …   Wikipedia

  • Immunoprécipitation — L immunoprécipitation (IP) est la technique qui permet la précipitation d un antigène (protéine) en solution par un anticorps qui agglutine spécifiquement une protéine particulière. On l utilise pour isoler et concentrer une protéine précise… …   Wikipédia en Français

  • Proteasome — Proteasomes are large protein complexes inside all eukaryotes and archaea, as well as in some bacteria. In eukaryotes, they are located in the nucleus and the cytoplasm.cite journal |author=Peters JM, Franke WW, Kleinschmidt JA |title=Distinct 19 …   Wikipedia

  • Survivin — Survivin, also called Baculoviral IAP repeat containing 5 (BIRC5), is a human gene that is part of the inhibitor of apoptosis family (IAP). The survivin protein functions to inhibit caspase activation therefore leading to negative regulation of… …   Wikipedia

  • Chromatin immunoprecipitation — The procedure of chromatin immunoprecipitation (ChIP) assay and methods of analysis Chromatin Immunoprecipitation (ChIP) is a type of immunoprecipitation experimental technique used to investigate the interaction between proteins and DNA in the… …   Wikipedia

  • Polyhistidine-tag — A polyhistidine tag is an amino acid motif in proteins that consists of at least six histidine ( His ) residues, often at the N or C terminus of the protein. It is also known as hexa histidine tag, 6xHis tag, and by the trademarked name His tag… …   Wikipedia

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